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KMID : 0848120120370030085
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International Journal of Oral Biology
2012 Volume.37 No. 3 p.85 ~ p.97
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Micro-CT analysis of LPS-induced Alveolar Bone Loss in Diabetic Mice
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Park Byung-Ju
Park Hae-Ryoung
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Abstract
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Periodontal disease induces an increased incidence oftooth loss, particularly in cases with an associated loss ofalveolar bone and periodontal ligaments. In this study,alveolar bone loss was detected by micro-computedtomography (CT) following exposure to E. coli lipopolysaccharide(LPS) in a streptozotocin (STZ)-induced diabeticmouse model. A 10 mg/ml dosage of E. coli LPS wasapplied between the first, second and third molars of themice three times a week for 10 weeks. The loss of periodontalligaments and alveolar processes was then evaluatedby micro-CT using two and three dimensional microstructuremorphometric parameters. In the diabetic mice, E.coli LPS induced the destruction of periodontal ligamentsand loss of alveolar process spaces. The distances betweenperiodontal ligaments were significantly widened in theSTZ-LPS group compared with the untreated STZ group.The 10 mg/ml exposure to E. coli LPS in the STZ mice alsoresulted in a significant decrease in the alveolar bonevolume fraction. The results of our study suggest thatalveolar bone loss can be readily detected by volumetricmicro-CT analysis as an increase in the distance betweenperiodontal ligaments and in the alveolar process length.
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KEYWORD
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lipopolysaccharide (LPS), diabetes, micro-CT, alveolar bone loss
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